kamalaker nasani | 12 Sep 09:40 2011
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Somatic embryogenesis of cotton

Dear members

I am working cotton on embryo genesis.  I am getting callus which looks like
globular stage embryos in my suspension cultures.  The problem is these
embryos are not proliferating further or converting into plants.

I have seen in one query that they are  using *picloramI or dicamba for
Maize suspension *cultures.  What is the role of these things in raising
somatic embryo suspension cultures.  Is it useful for other crops also.
 Please can I get any relevant literature regarding this.

If anybody working in Malvaceae family please let me know to discuss further
for assistance

--

-- 
Kamalaker Nasani,

 The experienced mountain climber is not intimidated by a mountain -- he is
inspired by it. The persistent winner is not discouraged by a problem -- he
is challenged by it. Mountains are created to be conquered; adversities are
designed to be defeated; problems are sent to be solved. It is better to
master one mountain than a thousand foothills.

-  William Arthur Wards
chovek69 | 13 Sep 10:20 2011
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Re: formamide for DNA

Try 1,5 Pentanediol... it has huge effect on DNA melting (can lower Tm
with more than 10C) and is absolutely non-toxic.

On Aug 19, 8:36 pm, Hiranya Roychowdhury <hroyc...@...> wrote:
> Sodium chloride ... (can be toxic in high enough concentrations, though ;))
>
> Hiranya S. Roychowdhury, Ph.D.
> Associate Professor
> Health & Public Services
> NMSU-Dona Ana Community  College
> 575 527 7725 (office)

>
> ________________________________________
> From: methods-boun...@...
[methods-boun...@...a.edu] on behalf of Ma,Jin [ji...@...]
> Sent: Friday, August 19, 2011 10:13 AM
> To: 'meth...@...'
> Subject: formamide for DNA
>
> Dear Sir./Madam,
> Do you know any other non or lower toxic chemicals which can attfect the Tm of DNA?
> Thanks.
> Jin Ma
> _______________________________________________
> Methods mailing list
> Meth...@...://www.bio.net/biomail/listinfo/methods
Virash Gupta | 15 Sep 12:52 2011
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Re: Methods Digest, Vol 76, Issue 2

what is the concentration of formamide and 1,5 Pentanediol to be used
in PCR for lowering melting temperature

On Wed, Sep 14, 2011 at 10:36 PM, 
<methods-request@...> wrote:
> Send Methods mailing list submissions to
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> Today's Topics:
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>   1. Re: formamide for DNA (chovek69)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 13 Sep 2011 01:20:57 -0700 (PDT)
> From: chovek69 <ivanoov@...>
(Continue reading)

PCR NEWS - September 2011 - focus on qPCR review papers

PCR NEWS - September 2011 - focus on qPCR review papers
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(Continue reading)

sudheer sangeetham | 22 Sep 18:11 2011
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Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Hi Guys
I am curious to know one thing that, I sterilised the LB media (which was
little more diluted). I inoculated the cultures with suitable antibiotics
and while I was measuring the OD of bacteria I could smell some medicine was
there from the media, I really dont understand why it was like that, but not
like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i
could nt able to express the protein.

The only mistake I did was LB media was little diluted, except this, I think
I have done everything right...

With curiosity, I would like to know what could be the problem? If any one
has any idea......

Thanks in advance

--

-- 
Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
Jayakumar, R | 22 Sep 20:24 2011

RE: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Sudheer,
   Medicine smell ?? Medicines smell differently.  Can you describe the smell better?  Did the colour of the
media change after you autoclaved it?  And how long did you autoclave it?

Jay

-----Original Message-----
From: methods-bounces@...
[mailto:methods-bounces@...] On Behalf Of
sudheer sangeetham
Sent: Thursday, September 22, 2011 12:12 PM
To: Methods@...
Subject: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Hi Guys
I am curious to know one thing that, I sterilised the LB media (which was
little more diluted). I inoculated the cultures with suitable antibiotics
and while I was measuring the OD of bacteria I could smell some medicine was
there from the media, I really dont understand why it was like that, but not
like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i
could nt able to express the protein.

The only mistake I did was LB media was little diluted, except this, I think
I have done everything right...

With curiosity, I would like to know what could be the problem? If any one
has any idea......

Thanks in advance

(Continue reading)

Prasad Senadheera | 24 Sep 04:30 2011
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Re: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

hi sudeer
probably the 'medicine smel'l may have come from the anibiotics or
some substances broken down or syntheisised by the fungus. we have
also used this media but have some type of strange smells after being
incubated with inoculations.

prasad

On Thu, Sep 22, 2011 at 11:54 PM, Jayakumar, R
<R.Jayakumar@...> wrote:
> Sudheer,
>   Medicine smell ?? Medicines smell differently.  Can you describe the smell better?  Did the colour
of the media change after you autoclaved it?  And how long did you autoclave it?
>
> Jay
>
>
> -----Original Message-----
> From: methods-bounces@...
[mailto:methods-bounces <at> oat.bio.indiana.edu] On Behalf Of sudheer sangeetham
> Sent: Thursday, September 22, 2011 12:12 PM
> To: Methods@...
> Subject: Sterilised LB media getting some medicine smell, while I was growing E.coli culture
>
> Hi Guys
> I am curious to know one thing that, I sterilised the LB media (which was
> little more diluted). I inoculated the cultures with suitable antibiotics
> and while I was measuring the OD of bacteria I could smell some medicine was
> there from the media, I really dont understand why it was like that, but not
> like earlier. After the OD was 0.55, i induced with IPTG but unfortunately i
(Continue reading)

sudheer sangeetham | 24 Sep 12:07 2011
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Re: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Hi Prasad
Thank you for the reply, may be your interpretation is right. In your case
when strange smells comes, could you able to express the protein?

Thank you in advance

Sudheer Babu.S
Ph.D student
Institute of Biochemistry
Biological Research Center
Szeged,Hungary.
WS | 24 Sep 22:57 2011
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Re: suggest me binding solution for SIGMA geneElut plant mini preparation kit.

Have you any leftovers? Probably, most important are pH and ionic 
strength. If you have the possibility, have it analyzed in a lab that 
does clinical urine analysis. They should be able to tell you what's 
inside.

HTH

Wo

 pls. suggest me binding solution for geneElut plant
> mini preparation kit.
> 
> 
>                                          kunal kale
WS | 24 Sep 23:08 2011
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Re: Sterilised LB media getting some medicine smell, while I was growing E.coli culture

Dear Sudheer,

honestly, I would safely discard the stuff (eg. add a gulp of bleach, 
incubate for a few hours and then autoclave it). When something unknown 
is growing, you don't know its effects on the result (in your case: 
protein expression) and more important, you might be growing a jar of 
some sort of unknown biohazard you wouldn't want to deal with. 

If this should happen repeatedly, maybe you want to have the bug 
identified by a department for microbiology. Any hospital lab should able 
to counsel you. And, of course, find out, where it comes from.

Having a short smell is something I also did when growing bacterial 
preps. A humsn's nose is a very good (fuzzy :) gas chromatograph.

One constellation comes into my mind where the smell might be ok:

Is your protein an enzyme? Then it might be that you simply smell what it 
converts the medium into... 

HTH & have fun!

Wo

At Sat, 24 Sep 2011 12:07:31 +0200, sudheer sangeetham wrote:

> Hi Prasad
> Thank you for the reply, may be your interpretation is right. In your
> case when strange smells comes, could you able to express the protein?
> 
(Continue reading)


Gmane