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Sharon Waldrop | 5 Oct 2010 01:23
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FW: Free siRNA Design software

Hello Everyone,

Does anyone know about this freeware that Marc Samet is talking about? We would both appreciate the siRNA
design freeware. Thank you for your help.

Sharon (Shar) Waldrop
Senior Research Associate
University of TX Southwestern Medical Center
Department of Internal Medicine - GI
Mail Code: 9151
5323 Harry Hines Blvd.
Dallas, TX 75390-9151
Dr. Gordan Kilic's Lab - Room# J05.114
Lab: 214-648-2063
Fax: 214-648-3088
Cell: 214-450-8086

________________________________________
From: Marc Samet [msamet1@...]
Sent: Monday, October 04, 2010 5:03 PM
To: Sharon Waldrop
Subject: RE: Free siRNA Design software

Never received any software

-----Original Message-----
From: Sharon Waldrop [mailto:Sharon.Waldrop@...]
Sent: Monday, October 04, 2010 5:49 PM
To: msamet1@...
Subject: FW: Free siRNA Design software
(Continue reading)

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David-Paul Minde | 12 Oct 2010 23:27
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Sypro Orange

>
> Dear all,
>
has anyone of you experience with Sypro Orange for protein gel staining and
(stoichiometric) quantifications ?
I read of it in a recent BRCA2 nature article but wonder why this technique
is not much more commonly applied for all kinds of in gel protein
quantifications. Excellent combination of huge linear range, lowest
protein-protein variability, silverlike sensitivity and utmost simplicity,
fairly reasonable price seems to be pretty much unique among protein gel
stains or did I overlook any important practical drawbacks ?
Many thanks for your comments in advance !
best,
David
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WS | 13 Oct 2010 08:00
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Re: Sypro Orange

Hi David,

several reasons:

- no equipment (illuminator, fluorescence scanner, camera set-up)
- price of dye, price of equipment
- used to "old methods" (coomassie, silver) which usually are
sufficient
- no need to quantify proteins in gel
- for real quantification, you'd always need a standard curve (dye
binding properties of each protein, co-absorbance, fluorescence
quenching)
- existing alternatives for specific quantification like western
blotting and eg antibody coated chips

- are you the product manager :)

suggest to cross-post in the ImageJ (http://en.wikipedia.org/wiki/
ImageJ) mailing list: IMAGEJ@...

/Wo

On Oct 12, 11:27 pm, David-Paul Minde <davidmi...@...> wrote:
> > Dear all,
>
> has anyone of you experience with Sypro Orange for protein gel staining and
> (stoichiometric) quantifications ?
> I read of it in a recent BRCA2 nature article but wonder why this technique
> is not much more commonly applied for all kinds of in gel protein
> quantifications. Excellent combination of huge linear range, lowest
(Continue reading)

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Editor www.Gene-Quantification.info | 27 Oct 2010 17:32

qPCR NEWS - October 2010 - focus on digital PCR

qPCR NEWS - October 2010 - focus on digital PCR
-----------------------------------------------------------------

Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

NEW - Copy Number Variations  => http://digital-PCR.gene-quantification.info
qPCR Symposium USA in November 2010
qPCR Application Workshops  -  and more ... ... ...

--------------------------------------------------------------------------------

Digital PCR (dPCR) is a refinement of conventional PCR methods that
can be used to directly quantify and clonally amplify nucleic acids
(including DNA, cDNA, methylated DNA, or RNA). The key difference
between dPCR and traditional PCR lies in the method of measuring
nucleic acids amounts, with the former being a more precise method
than PCR. The main principle of digital PCR is that a single sample is
split into many fractions, all of which are subsequently analysed by a
standard PCR method. The sample is fractionated by the simple process
of dilution so that each fraction contains approximately one copy of
DNA template. This separation allows a more reliable collection and
sensitive measurement of nucleic acid amounts. The other major feature
of digital PCR is that data are recorded as positive or negative (i.e.
generation of an amplification product or not). Hence, the individual
readout signals are qualitative or ‘digital’ in nature. The method has
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Mary | 28 Oct 2010 05:32
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Invitation to join the largest Cytogenetics discussion group on the Web

Hi,
 I would like to invite you to join the largest online cytogenetics
discussion group on the web.
 Its free to join. Its a friendly and helpfulgroup to
discuss: blood/bone marrow/solid tumour/prenatal/skins/POC cytogenetic
methodologies, as well as animal & plant cytogenetics including
molecular cytogenetics.
 Trouble-shooting all cytogenetic problems such as harvesting
problems, banding and cell culture.
Please invite others you feel would benefit from joining too.
Weblink:

http://groups.google.com.au/group/cytogenetics-methods-and-trouble-shooting
regards
Group Moderator
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Gmane